PMID:21383139

Citation	Fu, J, Munro, JB, Blanchard, SC and Frank, J (2011) Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation. Proc. Natl. Acad. Sci. U.S.A. 108:4817-21
Abstract	mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.
Links	PubMed PMC3064370 Online version:10.1073/pnas.1101503108
Keywords	Cryoelectron Microscopy/methods; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Mutation; Peptide Chain Elongation, Translational/physiology; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; RNA, Messenger/chemistry; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Transfer/chemistry; RNA, Transfer/genetics; RNA, Transfer/metabolism; Ribosomes/chemistry; Ribosomes/ultrastructure
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.