PMID:2138002
| Citation |
Pagan, J and Senior, AE (1990) Mutations in alpha-subunit of Escherichia coli F1-ATPase obtained by hydroxylamine-mutagenesis of plasmids carrying the uncA gene. Arch. Biochem. Biophys. 277:283-9 |
|---|---|
| Abstract |
In order to generate mutants randomly in the Escherichia coli uncA gene (encoding the alpha-subunit of F1-ATPase), plasmids carrying uncA were treated in vitro with hydroxylamine. Restriction fragments of the mutated uncA gene were then reconstructed into plasmid pDP34, which expresses all of the F1F0 structural genes, and the reconstructed mutant plasmids were expressed in a strain carrying a deletion of chromosomal uncA. Each of the mutations was characterized by DNA sequencing, growth assays, and biochemical assays of membrane preparations. Three nonsense and one frameshift mutation were identified and their properties were studied briefly. Eight new missense mutations were identified and characterization of their properties is described. These eight mutations were R139H, A177V, R210C, R303C, A306V, T343I, G351S, and P370L. |
| Links | |
| Keywords |
Amino Acid Sequence; Cell Membrane/enzymology; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial/drug effects; Genotype; Hydroxylamine; Hydroxylamines/pharmacology; Macromolecular Substances; Molecular Sequence Data; Mutation; Plasmids/drug effects; Protein Conformation; Proton-Translocating ATPases/genetics; Restriction Mapping; Temperature |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.