PMID:21378059
| Citation |
McCabe, KM, Lacherndo, EJ, Albino-Flores, I, Sheehan, E and Hernandez, M (2011) LacI(Ts)-regulated expression as an in situ intracellular biomolecular thermometer. Appl. Environ. Microbiol. 77:2863-8 |
|---|---|
| Abstract |
In response to needs for in situ thermometry, a temperature-sensitive vector was adapted to report changes in the intracellular heat content of Escherichia coli in near-real time. This model system utilized vectors expressing increasing quantities of β-galactosidase in response to stepwise temperature increases through a biologically relevant range (22 to 45°C). As judged by calibrated fluorometric and colorimetric reporters, both whole E. coli cells and lysates expressed significant repeatable changes in β-galactosidase activity that were sensitive to temperature changes of less than 1°C (35 to 45°C). This model system suggests that changes in cellular heat content can be detected independently of the medium in which cells are maintained, a feature of particular importance where the medium is heterogeneous or nonaqueous, or otherwise has a low heat transfer capacity. We report here that the intracellular temperature can be reliably obtained in near-real time using reliable fluorescent reporting systems from cellular scales, with a 20°C range of detection and at least 0.7°C sensitivity between 35 and 45°C. |
| Links |
PubMed PMC3126416 Online version:10.1128/AEM.01915-10 |
| Keywords |
Biosensing Techniques/methods; Colorimetry/methods; Escherichia coli/radiation effects; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Fluorometry/methods; Gene Expression Regulation, Bacterial; Lac Repressors/genetics; Lac Repressors/metabolism; Temperature; beta-Galactosidase/biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.