PMID:21371320
| Citation |
Menzella, HG (2011) Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli. Microb. Cell Fact. 10:15 |
|---|---|
| Abstract |
Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expression requires choosing from a vast number of possible DNA sequences and different codon optimization methods have been used in the past decade. Here, a comparative study of the two most common methods is presented using calf prochymosin as a model. |
| Links |
PubMed PMC3056764 Online version:10.1186/1475-2859-10-15 |
| Keywords |
Animals; Cattle; Chymosin/biosynthesis; Chymosin/genetics; Cloning, Molecular; Codon; Enzyme Precursors/biosynthesis; Enzyme Precursors/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Genetic Vectors/chemistry; Inclusion Bodies/metabolism; Protein Refolding; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics |
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