PMID:21370049
| Citation |
Wang, M, Rada, C and Neuberger, MS (2011) A high-throughput assay for DNA deaminases. Methods Mol. Biol. 718:171-84 |
|---|---|
| Abstract |
Most members of the AID/APOBEC family of polynucleotide deaminases can catalyse the deamination of cytosine to uracil in DNA. They thereby function as active DNA mutators. Here, we describe how bacterial papillation assays can be adapted to monitor the mutator activity of AID/APOBEC proteins and show how such papillation assays can be used as a high-throughput screen to identify AID variants with increased specific activity. It should also be possible to use papillation assays for the identification of novel DNA deaminases. |
| Links |
PubMed Online version:10.1007/978-1-61779-018-8_11 |
| Keywords |
Cytidine Deaminase/genetics; Cytidine Deaminase/metabolism; Cytosine/metabolism; DNA/genetics; DNA/metabolism; Escherichia coli/genetics; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; High-Throughput Screening Assays/methods; Mutation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.