PMID:2134185

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Citation

Yamada, M, Yamada, Y and Saier, MH Jr (1990) Nucleotide sequence and expression of the gutQ gene within the glucitol operon of Escherichia coli. DNA Seq. 1:141-5

Abstract

The glucitol operon in Escherichia coli is known to consist of five structural genes with the order: gut O P A B D M R. We have sequenced downstream from gutR and have identified an open reading frame encoding a water-soluble protein (223 amino acids; molecular weight = 23,562) with a putative ATP binding site. Expression of this protein in a maxicell system has been demonstrated. A repetitive extragenic palindromic (REP) sequence capable of forming stem-loop structures follows gutQ in the downstream, presumptive intercistronic region. The function of the Gut Q protein is not known.

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Keywords

Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; DNA, Bacterial; Escherichia coli/genetics; Escherichia coli Proteins; Molecular Sequence Data; Open Reading Frames; Operon; Restriction Mapping; Sorbitol

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