PMID:2113912

Citation	Steiert, JG, Rolfes, RJ, Zalkin, H and Stauffer, GV (1990) Regulation of the Escherichia coli glyA gene by the purR gene product. J. Bacteriol. 172:3799-803
Abstract	The purine regulon repressor protein, PurR, was shown to be a purine component involved in glyA regulation in Escherichia coli. Expression of glyA, encoding serine hydroxymethyltransferase activity, was elevated in a purR mutant compared with a wild-type strain. When the purR mutant was transformed with a plasmid carrying the purR gene, the serine hydroxymethyltransferase levels returned to the wild-type level. The PurR protein bound specifically to a DNA fragment carrying the glyA control region, as determined by gel retardation. In a DNase I protection assay, a 24-base-pair region was protected from DNase I digestion by PurR. The glyA operator sequence for PurR binding is similar to that reported for several pur regulon genes.
Links	PubMed PMC213358
Keywords	Base Sequence; Binding Sites; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Deoxyribonuclease I; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial; Glycine/biosynthesis; Glycine Hydroxymethyltransferase/genetics; Molecular Sequence Data; Mutation; Plasmids; Repressor Proteins/genetics; Repressor Proteins/metabolism; Serine/metabolism; Transcription Factors/genetics; Transduction, Genetic; Transferases/genetics
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