PMID:2113049
| Citation |
Schneider, GJ and Geiduschek, EP (1990) Stoichiometry of DNA binding by the bacteriophage SP01-encoded type II DNA-binding protein TF1. J. Biol. Chem. 265:10198-200 |
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| Abstract |
The stoichiometry of DNA binding by the bacteriophage SP01-encoded type II DNA-binding protein TF1 has been determined. 3H-Labeled TF1 was allowed to bind to a 32P-labeled DNA fragment containing a TF1 binding site. Multiple TF1-DNA complexes were resolved from each other and from unbound DNA by native gel electrophoresis. DNA-protein complexes were cut from polyacrylamide gels, and the amounts of 3H and 32P contained in each slice were measured. A ratio of 1.12 +/- 0.06 TF1 dimer/DNA molecule was calculated for the fastest-migrating TF1-DNA complex. We conclude that TF1 has a DNA-binding unit of one dimer. More slowly migrating complexes are apparently formed by serial addition of single TF1 dimers. |
| Links | |
| Keywords |
Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Bacteriophages/genetics; Bacteriophages/metabolism; Chromatography, Gel; DNA, Viral/genetics; DNA, Viral/isolation & purification; DNA, Viral/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/isolation & purification; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Kinetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism |
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