PMID:2108679
| Citation |
Tei, H, Murata, K and Kimura, A (1990) Structure and expression of cysX, the second gene in the Escherichia coli K-12 cysE locus. Biochem. Biophys. Res. Commun. 167:948-55 |
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| Abstract |
Protein products of the cysE region at 81 min on the chromosome of Escherichia coli K-12 (1) were analyzed by the maxicell method. Two kinds of polypeptides of molecular weight 33 K and 16 K were the products. The open reading frame (ORF) of the 33 K polypeptide consisted of 273 amino acids (Mr = 29,261). On the other hand, the 16 K ORF was overlapped by the opposite 33 K ORF and specified an extremely basic protein of 130 amino acids (Mr = 15,233). The gene coding for the 16 K polypeptide was named cysX. The expression of cysE and cysX in vivo was confirmed further by constructions of the cysE'-'lacZ and cysX'-'lacZ hybrid genes. |
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| Keywords |
Acetyltransferases/genetics; Acetyltransferases/metabolism; Amino Acid Sequence; Base Sequence; Cloning, Molecular/methods; Codon/genetics; Coliphages/genetics; Cysteine/biosynthesis; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Plasmids; Restriction Mapping; Serine O-Acetyltransferase; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
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