PMID:2104606
| Citation |
Lee, L and Imae, Y (1990) Role of threonine residue 154 in ligand recognition of the tar chemoreceptor in Escherichia coli. J. Bacteriol. 172:377-82 |
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| Abstract |
The Tar chemoreceptor of Escherichia coli mediates attractant responses to aspartate, maltose, and phenol, repellent responses to Ni2+ and Co2+, and thermoresponses. To understand the role of threonine residue 154, which is located in the ligand-binding domain of Tar, we replaced the residue with serine, isoleucine, and proline by site-directed mutagenesis. The replacements caused reductions in aspartate sensing but had only a small effect on maltose sensing and almost no effect on phenol sensing, repellent sensing, and thermosensing. These results indicate that Thr-154 of Tar is rather specifically involved in aspartate sensing. The reductions in the response threshold for aspartate by the replacements with serine, isoleucine, and proline were less than 1, about 2, and more than 5 orders of magnitude, respectively. When the corresponding threonine residue in the Tsr chemoreceptor was replaced with the same amino acids, roughly similar reductions in the response threshold for serine resulted. Thus, these threonine residues seem to have a common role in detecting the aspartate and serine attractant families. A mechanism by which these chemoreceptors detect the amino acid attractants is discussed. |
| Links | |
| Keywords |
Aspartic Acid/pharmacology; Bacterial Proteins; Chemoreceptor Cells; Chemotaxis; Escherichia coli/physiology; Escherichia coli Proteins; Hot Temperature; Maltose/pharmacology; Membrane Proteins; Methylation; Mutation; Phenols/pharmacology; Receptors, Cell Surface; Serine/pharmacology; Structure-Activity Relationship; Threonine/physiology |
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