PMID:20946386

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Citation

Smith, ML, Avanigadda, LN, Liddell, PW, Kenwright, KM and Howe, MM (2010) Identification of the J and K genes in the bacteriophage Mu genome sequence. FEMS Microbiol. Lett. 313:29-32

Abstract

Bacteriophage Mu was the first transposable phage to be discovered and still serves as the model for a large family of related transposable phages and prophages. The Mu genome sequence is known (NC-000929.1 GI:9633494), but not all of the genes have been assigned to the ORFs in the genome sequence. For this paper, we have sequenced an approximately 3-kb DNA region containing four predicted ORFs, Mup35-Mup38, from lysogens containing amber mutant prophages defective in either the J or the K gene. Amber mutations in prophages with J gene mutations mapped to the Mup36 ORF, and those in the K gene were found in Mup37, identifying the ORFs corresponding to these genes.

Links

PubMed Online version:10.1111/j.1574-6968.2010.02128.x

Keywords

Bacteriophage mu/genetics; Genes, Viral/genetics; Genome, Viral/genetics; Mutation; Open Reading Frames; Viral Tail Proteins/genetics

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