PMID:2089218
| Citation |
Kadner, RJ (1990) Vitamin B12 transport in Escherichia coli: energy coupling between membranes. Mol. Microbiol. 4:2027-33 |
|---|---|
| Abstract |
Cells of Escherichia coli possess high-affinity active transport systems of vitamin B12 and iron-siderophore complexes. Specific outer-membrane proteins carry out the energy-dependent transport across the outer membrane, in conjunction with the TonB coupling protein. Mutagenesis experiments have identified a conserved region near the amino-terminus of the outer-membrane transporters that is necessary for energy-coupled transport. The ability of extragenic suppressor mutations in tonB to correct the transport defect indicates that TonB couples the proton-motive force to the outer-membrane proteins by direct contact. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/metabolism; Biological Transport, Active; Calcium/metabolism; Energy Transfer; Escherichia coli/metabolism; Escherichia coli Proteins; Membrane Proteins/metabolism; Molecular Sequence Data; Mutagenesis; Vitamin B 12/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.