PMID:20659168
| Citation |
Sénéchal, H, Delesques, J and Szatmari, G (2010) Escherichia coli ArgR mutants defective in cer/Xer recombination, but not in DNA binding. FEMS Microbiol. Lett. 305:162-9 |
|---|---|
| Abstract |
The Escherichia coli arginine repressor (ArgR) is an L-arginine-dependent DNA-binding protein that controls the expression of the arginine biosynthetic genes and is required as an accessory factor for Xer site-specific recombination at cer and related recombination sites in plasmids. We used the technique of pentapeptide scanning mutagenesis to isolate a series of ArgR mutants that were considerably reduced in cer recombination, but were still able to repress an argA::lacZ fusion. DNA sequence analysis showed that all of the mutants mapped to the same nucleotide, resulting in a five amino acid insertion between residues 149 and 150 of ArgR, corresponding to the end of the alpha6 helix. A truncated ArgR containing a stop codon at residue 150 displayed the same phenotype as the protein with the five amino acid insertion, and both mutants displayed sequence-specific DNA-binding activity that was L-arginine dependent. These results show that the C-terminus of ArgR is more important in cer/Xer site-specific recombination than in DNA binding. |
| Links |
PubMed Online version:10.1111/j.1574-6968.2010.01921.x |
| Keywords |
Amino Acid Sequence; Chromosomes, Bacterial; Codon, Nonsense; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Mutant Proteins/genetics; Mutant Proteins/metabolism; Plasmids; Recombination, Genetic; Repressor Proteins/genetics; Repressor Proteins/metabolism; Sequence Alignment; Sequence Deletion |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.