PMID:2061278
| Citation |
Brendler, T, Abeles, A and Austin, S (1991) Critical sequences in the core of the P1 plasmid replication origin. J. Bacteriol. 173:3935-42 |
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| Abstract |
The core of the P1 plasmid replication origin consists of a series of 7-bp repeats and a G+C-rich stretch. Methylation of the GATC sequences in the repeats is essential. Forty different single-base mutations in the region were isolated and assayed for origin function. A single-base change within any 7-bp repeat could block the origin, irrespective of whether GATC bases were affected. The repeats themselves were critical, but the short intervals between them were not. Mutations in the G+C-rich region showed it to be a spacer whose exact length is important but whose sequence can vary considerably. It maintains a precise distance between the 7-bp repeats and binding sites for the P1 RepA initiator protein. It may also serve as a clamp to limit strand separation during initiation. |
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| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacteriophages/genetics; Base Composition; Base Sequence; DNA Helicases; DNA Replication; DNA, Viral/biosynthesis; DNA, Viral/chemistry; DNA-Binding Proteins; Methylation; Molecular Sequence Data; Mutagenesis; Phenotype; Plasmids; Proteins; Repetitive Sequences, Nucleic Acid; Replicon; Trans-Activators |
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