PMID:2050643
| Citation |
Zheng, L and Braymer, HD (1991) Overproduction and purification of McrC protein from Escherichia coli K-12. J. Bacteriol. 173:3918-20 |
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| Abstract |
The McrC protein, encoded by one of the two genes involved in the McrB restriction system, was produced in Escherichia coli cells by using a T7 expression system. Following sequential DEAE-Sepharose and hydroxylapatite column chromatography, the protein was purified to apparent homogeneity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of the purified McrC protein agreed exactly with the one deduced from the DNA sequence by Ross et al. (J. Bacteriol. 171:1974-1981, 1989). |
| Links | |
| Keywords |
Autoradiography; Bacterial Proteins/biosynthesis; Bacterial Proteins/isolation & purification; DNA Restriction Enzymes; DNA-Cytosine Methylases/genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli/metabolism; Escherichia coli Proteins; Open Reading Frames; Plasmids; Restriction Mapping |
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