PMID:20436399

Citation	*Pomerantz, RT and O'Donnell, M' (2010) Direct restart of a replication fork stalled by a head-on RNA polymerase.* J Vis Exp '**
Abstract	In vivo studies suggest that replication forks are arrested due to encounters with head-on transcription complexes. Yet, the fate of the replisome and RNA polymerase (RNAP) following a head-on collision is unknown. Here, we find that the E. coli replisome stalls upon collision with a head-on transcription complex, but instead of collapsing, the replication fork remains highly stable and eventually resumes elongation after displacing the RNAP from DNA. We also find that the transcription-repair coupling factor, Mfd, promotes direct restart of the fork following the collision by facilitating displacement of the RNAP. These findings demonstrate the intrinsic stability of the replication apparatus and a novel role for the transcription-coupled repair pathway in promoting replication past a RNAP block.
Links	PubMed PMC3146238 Online version:10.3791/1919
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA Replication; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/genetics; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic
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