PMID:20435136
| Citation |
Krin, E, Danchin, A and Soutourina, O (2010) RcsB plays a central role in H-NS-dependent regulation of motility and acid stress resistance in Escherichia coli. Res. Microbiol. 161:363-71 |
|---|---|
| Abstract |
In Escherichia coli, hns mutants lack flagellar motility and display an increase in acid stress resistance. Spontaneous phenotypic revertants showed reversion of both H-NS-controlled phenotypes. In the present study, suppressor mutations were identified in the rcsB gene. In addition to RcsA, our experiments establish that H-NS indirectly controlled the RcsB regulator via repression of RcsD. We also show that RcsB(D56E), mimicking phosphorylated RcsB, interacts with GadE to form a RcsB-P/GadE complex, a general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways. In addition, we showed that H-NS positively affects motility via the flhDC master operon repression by RcsB. This substantiates the central role of RcsB in H-NS-mediated control of motility and acid stress resistance. |
| Links |
PubMed Online version:10.1016/j.resmic.2010.04.002 |
| Keywords |
Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Fimbriae Proteins/genetics; Fimbriae Proteins/metabolism; Flagella/genetics; Flagella/metabolism; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Molecular Sequence Data; Movement; Operon; Phosphorylation; Phosphotransferases/genetics; Phosphotransferases/metabolism; Regulatory Elements, Transcriptional; Stress, Physiological; Suppression, Genetic; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism |
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