PMID:20378962
| Citation |
Kashihara, H, Kang, BM, Omasa, T, Honda, K, Sameshima, Y, Kuroda, A and Ohtake, H (2010) Electron microscopic analysis of heat-induced leakage of polyphosphate from a phoU mutant of Escherichia coli. Biosci. Biotechnol. Biochem. 74:865-8 |
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| Abstract |
Heat treatment is a simple technique for releasing polyphosphate (polyP) from microbial cells. We investigated the ultrastructural alterations of polyP granules (PPGs) in response to heat treatment at 70 degrees C using a polyP-accumulating phoU mutant of Escherichia coli as a model organism. Electron microscopic and energy-dispersive X-ray analysis suggested concurrent occurrence of PPG degradation and polyP release in the cytoplasm. PolyP is probably released by cytoplasmic extrusion through breaks in the cell envelope. |
| Links | |
| Keywords |
Cell Membrane/genetics; Cell Membrane/metabolism; Electrons; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Hot Temperature; Microscopy, Electron; Polyphosphates/chemistry; Polyphosphates/metabolism; X-Rays |
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