PMID:20364292
| Citation |
Doroshenko, VG, Shakulov, RS, Kazakova, SM, Kivero, AD, Yampolskaya, TA and Mashko, SV (2010) Construction of an L-phenylalanine-producing tyrosine-prototrophic Escherichia coli strain using tyrA ssrA-like tagged alleles. Biotechnol. Lett. 32:1117-21 |
|---|---|
| Abstract |
To construct a Phe-producing Tyr(+) Escherichia coli strain, TyrA (chorismate mutase/prephenate dehydrogenase) activity was varied by engineering a proteolytically unstable protein. The tyrA in the E. coli BW25113 was altered to include ssrA-like tags. The tagged tyrA genes, which ensured different growth rates in M9 medium, were introduced into a Phe-producing strain to replace DeltatyrA. Strains with unstable TyrA-(A)ANDENYALAA proteins had a lower biomass yield and a higher Phe accumulation than strains generating the more stable TyrA-(A)ANDENYALDD. The Tyr/Phe ratio produced by the TyrA-tag strains was 10-fold less than that produced by the TyrA(wt) strain. |
| Links |
PubMed Online version:10.1007/s10529-010-0265-1 |
| Keywords |
Amino Acid Sequence; Chromatography, High Pressure Liquid; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Molecular Sequence Data; Phenylalanine/analysis; Phenylalanine/biosynthesis; Prephenate Dehydrogenase/genetics; Prephenate Dehydrogenase/metabolism; Time Factors; Tyrosine/analysis; Tyrosine/genetics |
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