PMID:2034225

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Citation

Ben-Neria, T and Ron, EZ (1991) A DNA replication gene maps near terC in Escherichia coli K12. Mol. Gen. Genet. 226:315-7

Abstract

Temperature-sensitive mutants that filamented at the non-permissive temperature were isolated by specific mutagenesis of the terminus region of the Escherichia coli chromosome. Two of them, mapping at about 35 min, failed to divide due to inhibition of DNA replication. Further characterization indicated that these mutants are temperature-sensitive for DNA chain elongation.

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Keywords

Chromosome Mapping; Chromosomes, Bacterial; DNA Replication/genetics; DNA, Bacterial/biosynthesis; DNA, Bacterial/genetics; Escherichia coli/genetics; Mutagenesis; Nucleic Acid Hybridization; Temperature; Terminator Regions, Genetic/genetics

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