PMID:20233924
| Citation |
Lukas, H, Reimann, J, Kim, OB, Grimpo, J and Unden, G (2010) Regulation of aerobic and anaerobic D-malate metabolism of Escherichia coli by the LysR-type regulator DmlR (YeaT). J. Bacteriol. 192:2503-11 |
|---|---|
| Abstract |
Escherichia coli K-12 is able to grow under aerobic conditions on D-malate using DctA for D-malate uptake and the D-malate dehydrogenase DmlA (formerly YeaU) for converting D-malate to pyruvate. Induction of dmlA encoding DmlA required an intact dmlR (formerly yeaT) gene, which encodes DmlR, a LysR-type transcriptional regulator. Induction of dmlA by DmlR required the presence of D-malate or L- or meso-tartrate, but only D-malate supported aerobic growth. The regulator of general C(4)-dicarboxylate metabolism (DcuS-DcuR two-component system) had some effect on dmlA expression. The anaerobic L-tartrate regulator TtdR or the oxygen sensors ArcB-ArcA and FNR did not have a major effect on dmlA expression. DmlR has a high level of sequence identity (49%) with TtdR, the L- and meso-tartrate-specific regulator of L-tartrate fermentation in E. coli. dmlA was also expressed at high levels under anaerobic conditions, and the bacteria had D-malate dehydrogenase activity. These bacteria, however, were not able to grow on D-malate since the anaerobic pathway for D-malate degradation has a predicted yield of < or = 0 ATP/mol D-malate. Slow anaerobic growth on D-malate was observed when glycerol was also provided as an electron donor, and D-malate was used in fumarate respiration. The expression of dmlR is subject to negative autoregulation. The network for regulation and coordination of the central and peripheral pathways for C(4)-dicarboxylate metabolism by the regulators DcuS-DcuR, DmlR, and TtdR is discussed. |
| Links |
PubMed PMC2863561 Online version:10.1128/JB.01665-09 |
| Keywords |
Aerobiosis/genetics; Aerobiosis/physiology; Anaerobiosis/genetics; Anaerobiosis/physiology; Chromatography, High Pressure Liquid; Escherichia coli K12/genetics; Escherichia coli K12/growth & development; Escherichia coli K12/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial/genetics; Gene Expression Regulation, Bacterial/physiology; Malate Dehydrogenase/genetics; Malate Dehydrogenase/metabolism; Malates/metabolism; Mutation; Tartrates/metabolism; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
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