PMID:20228083
| Citation |
Johnsen, L, Weigel, C, von Kries, J, Møller, M and Skarstad, K (2010) A novel DNA gyrase inhibitor rescues Escherichia coli dnaAcos mutant cells from lethal hyperinitiation. J. Antimicrob. Chemother. 65:924-30 |
|---|---|
| Abstract |
In order to search for novel antibacterial compounds we used a previously developed screening strain designed specifically to discover inhibitors of the bacterial initiator protein, DnaA. This strain (SF53) is not viable at 30 degrees C due to overinitiation. Therefore, compounds that are able to restore growth to SF53 cells are likely to cause either partial or complete inhibition of DnaA function. In this study we used SF53 cells to screen the Library of Pharmacologically Active Compounds (LOPAC). |
| Links |
PubMed Online version:10.1093/jac/dkq071 |
| Keywords |
Bacterial Proteins/genetics; Benzazepines/pharmacology; DNA Gyrase/antagonists & inhibitors; DNA-Binding Proteins/genetics; Drug Evaluation, Preclinical/methods; Enzyme Inhibitors/pharmacology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins/antagonists & inhibitors |
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