PMID:20217281
| Citation |
Joseph, TC, Rajan, LA, Thampuran, N and James, R (2010) Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance. Mol. Biotechnol. 46:20-5 |
|---|---|
| Abstract |
Trehalose (1-alpha-D-glucopyranosyl-1-alpha-D-glucopyranoside), a non-reducing disaccharide is a major compatible solute, which maintains fluidity of membranes and protects the biological structure of organisms under stress. In this study, trehalose-6-phosphate synthase (otsA) and trehalose-6-phosphate phosphatase (otsB) genes encoding for trehalose biosynthesis from Escherichia coli was cloned as an operon and expressed in E. coli M15(pREP4). The recombinant E. coli strain showed a threefold increase in the activity of otsBA pathway enzymes, compared to the control strain. The transgenic E. coli accumulated up to 0.86 mg/l of trehalose. The sequence of otsA and otsB genes reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. |
| Links |
PubMed Online version:10.1007/s12033-010-9259-4 |
| Keywords |
Adaptation, Physiological/genetics; Amino Acid Sequence; Amino Acid Substitution/genetics; Amino Acids/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Cloning, Molecular; Escherichia coli/genetics; Genes, Bacterial/genetics; Molecular Sequence Data; Osmolar Concentration; Polymerase Chain Reaction; Protein Biosynthesis/genetics; Sequence Analysis, DNA; Stress, Physiological/genetics; Trehalose/biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.