PMID:20172997

Citation	Hwang, J and Inouye, M (2010) Interaction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain. J. Bacteriol. 192:2277-83
Abstract	Der, an essential Escherichia coli tandem GTPase, has been implicated in 50S subunit biogenesis. The rrmJ gene encodes a methyltransferase that modifies the U2552 residue of 23S rRNA, and its deletion causes a severe growth defect. Peculiarly, overexpression of Der suppresses growth impairment. In this study, using an rrmJ-deletion strain, we demonstrated that two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain. We also identified a region of Der that is critical for its specific interaction with 50S subunit.
Links	PubMed PMC2849445 Online version:10.1128/JB.00045-10
Keywords	Cell Cycle Proteins/genetics; Cell Cycle Proteins/physiology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; GTP-Binding Proteins/genetics; GTP-Binding Proteins/metabolism; Methyltransferases/genetics; Methyltransferases/physiology; Mutagenesis; Phenotype; Protein Binding/genetics; Protein Binding/physiology; Protein Structure, Tertiary/genetics; Protein Structure, Tertiary/physiology; Ribosome Subunits, Large, Bacterial/genetics; Ribosome Subunits, Large, Bacterial/metabolism
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