PMID:2016302
| Citation |
Ninfa, AJ and Bennett, RL (1991) Identification of the site of autophosphorylation of the bacterial protein kinase/phosphatase NRII. J. Biol. Chem. 266:6888-93 |
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| Abstract |
Previous studies have established that the Escherichia coli protein kinase/phosphatase nitrogen regulator II (NRII also known as NtrB) becomes autophosphorylated on a histidine residue when incubated with ATP. We show that the major site at which NRII was autophosphorylated was contained within a peptide consisting of amino acid residues 136-142 of NRII, and thus probably corresponds to His-139. A minor site of phosphorylation, accounting for about 2% of the phosphate in NRII-P, was found in a peptide that corresponds to residues 158-169. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Chromatography, High Pressure Liquid; DNA-Binding Proteins/isolation & purification; DNA-Binding Proteins/metabolism; Escherichia coli/enzymology; Molecular Sequence Data; PII Nitrogen Regulatory Proteins; Peptide Fragments/isolation & purification; Phosphopeptides/isolation & purification; Phosphoprotein Phosphatases; Phosphorylation; Protein Kinases; Trans-Activators; Transcription Factors; Trypsin |
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