PMID:20137911
| Citation |
Zhou, H, Liao, X, Wang, T, Du, G and Chen, J (2010) Enhanced l-phenylalanine biosynthesis by co-expression of pheA(fbr) and aroF(wt). Bioresour. Technol. 101:4151-6 |
|---|---|
| Abstract |
A beta-2-thienylalanine-resistant E. coli K12 mutant carrying a Thr326Pro mutation in the regulation (R) domain of pheA (pheA(fbr)) was obtained by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis. In the presence of 200mM l-phenylalanine (l-Phe), a recombinant E. coli WSH-Z06 (pAP-B03) carrying pheA(fbr) as well as wild-type aroF (aroF(wt)) exhibited more than 70% of the chorismate mutase-prephenate dehydratase (CM-PDT) activity as observed in the absence of this amino acid. The l-Phe titer of WSH-Z06 (pAP-B03) reached 35.38g/L in a 3-L fermentor, which was 2.81-fold higher than that of the original strain E. coli WSH-Z06. Furthermore, the l-Phe yield on glucose of WSH-Z06 (pAP-B03) (0.26mol/mol) was twice that of E. coli WSH-Z06. This recombinant E. coli WSH-Z06 (pAP-B03) is a potential strain for over-production of l-Phe. |
| Links |
PubMed Online version:10.1016/j.biortech.2010.01.043 |
| Keywords |
Escherichia coli/genetics; Escherichia coli/metabolism; Fermentation; Phenylalanine/biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.