PMID:20110508

Citation	Pomerantz, RT and O'Donnell, M (2010) Direct restart of a replication fork stalled by a head-on RNA polymerase. Science 327:590-2
Abstract	In vivo studies suggest that replication forks are arrested by encounters with head-on transcription complexes. Yet, the fate of the replisome and RNA polymerase (RNAP) after a head-on collision is unknown. We found that the Escherichia coli replisome stalls upon collision with a head-on transcription complex, but instead of collapsing, the replication fork remains highly stable and eventually resumes elongation after displacing the RNAP from DNA. We also found that the transcription-repair coupling factor Mfd promotes direct restart of the fork after the collision by facilitating displacement of the RNAP. These findings demonstrate the intrinsic stability of the replication apparatus and a previously unknown role for the transcription-coupled repair pathway in promoting replication past a RNAP block.
Links	PubMed PMC2861996 Online version:10.1126/science.1179595
Keywords	Bacterial Proteins/metabolism; DNA Repair; DNA Replication; DNA, Bacterial/metabolism; DNA-Directed DNA Polymerase/metabolism; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Templates, Genetic; Transcription Factors/metabolism; Transcription, Genetic
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