PMID:20075920
| Citation |
Epshtein, V, Dutta, D, Wade, J and Nudler, E (2010) An allosteric mechanism of Rho-dependent transcription termination. Nature 463:245-9 |
|---|---|
| Abstract |
Rho is the essential RNA helicase that sets the borders between transcription units and adjusts transcriptional yield to translational needs in bacteria. Although Rho was the first termination factor to be discovered, the actual mechanism by which it reaches and disrupts the elongation complex (EC) is unknown. Here we show that the termination-committed Rho molecule associates with RNA polymerase (RNAP) throughout the transcription cycle; that is, it does not require the nascent transcript for initial binding. Moreover, the formation of the RNAP-Rho complex is crucial for termination. We show further that Rho-dependent termination is a two-step process that involves rapid EC inactivation (trap) and a relatively slow dissociation. Inactivation is the critical rate-limiting step that establishes the position of the termination site. The trap mechanism depends on the allosterically induced rearrangement of the RNAP catalytic centre by means of the evolutionarily conserved mobile trigger-loop domain, which is also required for EC dissociation. The key structural and functional similarities, which we found between Rho-dependent and intrinsic (Rho-independent) termination pathways, argue that the allosteric mechanism of termination is general and likely to be preserved for all cellular RNAPs throughout evolution. |
| Links |
PubMed PMC2929367 Online version:10.1038/nature08669 |
| Keywords |
Allosteric Regulation; Binding Sites; Biocatalysis; Catalytic Domain; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Dicarboxylic Acids/pharmacology; Escherichia coli/enzymology; Kinetics; Mutant Proteins/genetics; Mutant Proteins/metabolism; Mutation/genetics; Organophosphorus Compounds/pharmacology; Protein Binding; Rho Factor/metabolism; Templates, Genetic; Transcription, Genetic/drug effects; Transcription, Genetic/physiology |
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