PMID:20072783
| Citation |
Connor, MR, Cann, AF and Liao, JC (2010) 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. Appl. Microbiol. Biotechnol. 86:1155-64 |
|---|---|
| Abstract |
Interest in producing biofuels from renewable sources has escalated due to energy and environmental concerns. Recently, the production of higher chain alcohols from 2-keto acid pathways has shown significant progress. In this paper, we demonstrate a mutagenesis approach in developing a strain of Escherichia coli for the production of 3-methyl-1-butanol by leveraging selective pressure toward L-leucine biosynthesis and screening for increased alcohol production. Random mutagenesis and selection with 4-aza-D,L-leucine, a structural analogue to L-leucine, resulted in the development of a new strain of E. coli able to produce 4.4 g/L of 3-methyl-1-butanol. Investigation of the host's sensitivity to 3-methyl-1-butanol directed development of a two-phase fermentation process in which titers reached 9.5 g/L of 3-methyl-1-butanol with a yield of 0.11 g/g glucose after 60 h. |
| Links |
PubMed PMC2844964 Online version:10.1007/s00253-009-2401-1 |
| Keywords |
Biofuels/microbiology; Escherichia coli/metabolism; Fermentation; Leucine/metabolism; Mutagenesis; Pentanols/metabolism; Selection, Genetic |
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