PMID:2005873
| Citation |
Krone, FA, Westphal, G and Schwenn, JD (1991) Characterisation of the gene cysH and of its product phospho-adenylylsulphate reductase from Escherichia coli. Mol. Gen. Genet. 225:314-9 |
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| Abstract |
The nucleotide sequence of the gene cysH from Escherichia coli K12 was determined. The open reading frame was 735 nucleotides in length; it was flanked by a repetitive palindromic sequence centred 36 nucleotides upstream of cysH and a terminator-like structure located 20 nucleotides downstream. CysH encoded a polypeptide of Mr 27927 consisting of 244 amino acids. The gene product was isolated as a homodimer exhibiting phospho-adenylylsulphate reductase (PAPS reductase) activity. The active enzyme was devoid of electron transferring cofactors and contained only one cysteine per subunit. Reduction of the enzyme by dithiols resulted in a shift of the apparent molecular weight from 44,000 to 62,000 without formation of an enzyme-thioredoxin complex. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Molecular Sequence Data; Molecular Weight; Open Reading Frames; Oxidoreductases/genetics; Oxidoreductases/metabolism; Phenotype; Repetitive Sequences, Nucleic Acid; Restriction Mapping |
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