PMID:1995431
| Citation |
Nègre, D, Cortay, JC, Old, IG, Galinier, A, Richaud, C, Saint Girons, I and Cozzone, AJ (1991) Overproduction and characterization of the iclR gene product of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2. Gene 97:29-37 |
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| Abstract |
The iclR gene of Escherichia coli K-12, which encodes a regulatory protein (repressor) for the aceBAK operon, is located between that operon and metH in the 91-min region of the chromosome. The iclR gene was cloned and expressed in a coupled T7 RNA polymerase/promoter system and the gene product was identified by specific binding to a fragment containing the aceBAK operator region. The iclR gene product is a polypeptide of 274 amino acids (aa) with a calculated Mr of 29,741. Comparison of the deduced IclR aa sequence to that of Salmonella typhimurium revealed that the two IclR repressors exhibit 89% identity. A possible helix-turn-helix motif characteristic of DNA-binding proteins was found within the IclR sequence. A search in protein data banks revealed that IclR has a score of similarity of 43.7% with GylR, a transcriptional regulator of the glycerol operon of Streptomyces coelicolor. |
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| Keywords |
Acetates/metabolism; Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Cloning, Molecular; DNA, Bacterial/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Regulator; Molecular Sequence Data; Operator Regions, Genetic; Operon; Repressor Proteins/biosynthesis; Repressor Proteins/genetics; Repressor Proteins/metabolism; Restriction Mapping; Salmonella typhimurium/genetics; Salmonella typhimurium/metabolism; Sequence Homology, Nucleic Acid; Transcription Factors |
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