PMID:19892204
| Citation |
Burgess, RR (2009) Important but little known (or forgotten) artifacts in protein biochemistry. Meth. Enzymol. 463:813-20 |
|---|---|
| Abstract |
Many subtle and sometimes obscure artifacts exist that can have major effects on the outcomes of otherwise carefully performed experiments. This chapter focuses on a few of these artifacts involved in processes such as SDS gel sample preparation, buffers, Ni-chelate affinity and ion-exchange chromatography, urea preparation, use of plasticware, and filtration of protein samples. |
| Links |
PubMed Online version:10.1016/S0076-6879(09)63044-5 |
| Keywords |
Absorption/physiology; Animals; Artifacts; Biochemistry/methods; Biochemistry/standards; Buffers; Chromatography/methods; Electrophoresis, Polyacrylamide Gel; Humans; Plastics/chemistry; Proteins/chemistry; Proteins/isolation & purification |
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