PMID:19848414
| Citation |
Brown, SD, Fiedler, JD and Finn, MG (2009) Assembly of hybrid bacteriophage Qbeta virus-like particles. Biochemistry 48:11155-7 |
|---|---|
| Abstract |
Bacteriophage Qbeta coat protein forms uniform virus-like particles when expressed recombinantly in a variety of organisms. We have inserted the IgG-binding Z domain at the carboxy terminus of the coat protein and coexpressed this chimeric subunit with native coat protein to create hybrid, IgG-binding virus-like particles. Extracellular osmolytes were found to have an effect on the efficiency of incorporation of fusion proteins into VLPs in Escherichia coli when a carbenicillin, but not a kanamycin, selection marker was used. The addition of sucrose to the growth medium decreased the incorporation efficiency; the osmoprotectant glycine betaine eliminated this effect. The decrease in efficiency was not observed when carbenicillin was omitted from the final expression culture. The addition of sodium chloride instead of sucrose gave rise to particles with a larger number of fusion proteins than the standard conditions. These results illustrate that cellular conditions should be taken into account even in apparently simple systems when natural or engineered protein nanoparticles are made. |
| Links |
PubMed PMC2799296 Online version:10.1021/bi901306p |
| Keywords |
Allolevivirus/genetics; Allolevivirus/metabolism; Allolevivirus/ultrastructure; Betaine/chemistry; Betaine/metabolism; Binding Sites; Capsid Proteins/metabolism; Carbenicillin/pharmacology; Escherichia coli/drug effects; Extracellular Space/metabolism; Immunoglobulin G/metabolism; Kanamycin/pharmacology; Lipotropic Agents/chemistry; Lipotropic Agents/metabolism; Sodium Chloride/chemistry; Sucrose/chemistry; Surface Properties; Virus Assembly |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.