PMID:1973523

Citation	Sanangelantoni, AM, Barbarini, D, Di Pasquale, G, Cammarano, P and Tiboni, O (1990) Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene: phylogenetic implications. Mol. Gen. Genet. 221:187-94
Abstract	The glnA gene of the thermophilic sulphur-dependent archaebacterium Sulfolobus solfataricus was identified by hybridization with the corresponding gene of the cyanobacterium Spirulina platensis and cloned in Escherichia coli. The nucleotide sequence of the 1696 bp DNA fragment containing the structural gene for glutamine synthetase was determined, and the derived amino acid sequence (471 residues) was compared to the sequences of glutamine synthetases from eubacteria and eukaryotes. The homology between the archaebacterial and the eubacterial enzymes is higher (42%-49%) than that found with the eukaryotic counterpart (less than 20%). This was true also when the five most conserved regions, which it is possible to identify in both eubacterial and eukaryotic glutamine synthetases, were analysed.
Links	PubMed
Keywords	Amino Acid Sequence; Archaea/enzymology; Archaea/genetics; Bacteria/genetics; Base Sequence; Cloning, Molecular; Codon; Genes, Bacterial; Glutamate-Ammonia Ligase/genetics; Glutamate-Ammonia Ligase/metabolism; Molecular Sequence Data; Phylogeny; Restriction Mapping; Sequence Homology, Nucleic Acid
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