PMID:1964171
| Citation |
Ratnaningsih, E, Dharmsthiti, S, Krishnapillai, V, Morgan, A, Sinclair, M and Holloway, BW (1990) A combined physical and genetic map of Pseudomonas aeruginosa PAO. J. Gen. Microbiol. 136:2351-7 |
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| Abstract |
A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome. |
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| Keywords |
Blotting, Southern; Cloning, Molecular; Cosmids; DNA Transposable Elements; Deoxyribonucleases, Type II Site-Specific; Electrophoresis, Agar Gel; Genes, Bacterial; Genetic Complementation Test; Genomic Library; Pseudomonas aeruginosa/genetics; Restriction Mapping |
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