PMID:1956285
| Citation |
Lamark, T, Kaasen, I, Eshoo, MW, Falkenberg, P, McDougall, J and Strøm, AR (1991) DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli. Mol. Microbiol. 5:1049-64 |
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| Abstract |
The sequence was determined of 6493 nucleotides encompassing the bet genes of Escherichia coli which encode the osmoregulatory choline-glycine betaine pathway. Four open reading frames were identified: betA encoding choline dehydrogenase, a flavoprotein of 61.9kDa; betB encoding betaine aldehyde dehydrogenase (52.8kDa); betT encoding a proton-motive-force-driven, high-affinity transport system for choline (75.8kDa); and betl, capable of encoding a protein of 21.8kDa, implicated as a repressor involved in choline regulation of the bet genes. Identification of the genes was supported by subcloning, physical mapping of lambda placMu53 insertions, amino acid sequence similarity, or N-terminal amino acid sequencing. The bet genes are tightly spaced, with betT located upstream of, and transcribed divergently to, the tandemly linked betIBA genes. |
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| Keywords |
Alcohol Oxidoreductases/genetics; Alcohol Oxidoreductases/metabolism; Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Betaine-Aldehyde Dehydrogenase; Biological Transport; Choline/metabolism; Choline Dehydrogenase; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Glycine/metabolism; Molecular Sequence Data; Open Reading Frames; Osmotic Pressure; Recombinant Fusion Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Sequence Homology, Nucleic Acid; Transcription, Genetic |
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