PMID:1955875

Citation	Douglas, RM, Roberts, JA, Munro, AW, Ritchie, GY, Lamb, AJ and Booth, IR (1991) The distribution of homologues of the Escherichia coli KefC K(+)-efflux system in other bacterial species. J. Gen. Microbiol. 137:1999-2005
Abstract	Using a variety of techniques the distribution of the glutathione-regulated KefC K(+)-transport system among bacterial species was investigated. The presence of similar systems in a number of Gram-negative bacteria was demonstrated. In contrast, the system appeared to be absent from most Gram-positive bacteria tested with the exception of Staphylococcus aureus. Using the cloned Escherichia coli kefC gene as a probe for Southern hybridization it was shown that only limited DNA sequence homology exists with other bacteria, even when closely related members of the enteric group were examined.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biological Transport, Active/drug effects; Blotting, Southern; Cloning, Molecular; DNA Probes/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Ethylmaleimide/pharmacology; Glutathione/analogs & derivatives; Glutathione/analysis; Glutathione/metabolism; Gram-Negative Bacteria/metabolism; Gram-Positive Bacteria/metabolism; Kinetics; Potassium/metabolism; Potassium Channels; Sequence Homology, Nucleic Acid; Staphylococcus aureus/metabolism; Succinimides/analysis
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