PMID:1938927
| Citation |
Hiom, K and Sedgwick, SG (1991) Cloning and structural characterization of the mcrA locus of Escherichia coli. J. Bacteriol. 173:7368-73 |
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| Abstract |
Escherichia coli has DNA restriction systems which are able to recognize and attack modified cytosine residues in the DNA of incoming bacteriophages and plasmids. The locus for the McrA/RglA system of modified cytosine restriction was located near the pin gene of the defective element, e14. Hence, loss of the e14 element through abortive induction after UV irradiation caused a permanent loss of McrA restriction activity. e14 DNA encoding McrA restriction was cloned and sequenced to reveal a single open reading frame of 831 bp with a predicted gene product of 31 kDa. Clones expressing the complete open reading frame conferred both McrA and RglA phenotypes; however, a deletion derivative was found which complemented RglA restriction against nonglucosylated T6gt phage but did not complement for McrA restriction of methylated plasmid DNA. Possible explanations for this activity and a comparison with the different organization of the McrB/RglB restriction system are discussed. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular/methods; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/radiation effects; Genes, Bacterial; Genetic Complementation Test; Molecular Sequence Data; Phenotype; Plasmids; Restriction Mapping; Transformation, Bacterial/radiation effects; Ultraviolet Rays |
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