PMID:1938906
| Citation |
Stolz, B and Berg, HC (1991) Evidence for interactions between MotA and MotB, torque-generating elements of the flagellar motor of Escherichia coli. J. Bacteriol. 173:7033-7 |
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| Abstract |
Cells that overexpress MotA (encoded on a plasmid derived from pBR322) grow slowly because of proton leakage. We have traced this defect to the coexpression of a fusion protein consisting of 60 amino acids from the N terminus of MotB and 50 amino acids specified by pBR322. Mutations within the N terminus, known to abolish function when present in full-length MotB, reversed the growth defect. Growth also was normal when MotA was coexpressed with wild-type MotB or with a series of MotB N-terminal fragments. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli/metabolism; Flagella/metabolism; Gene Expression; Genetic Complementation Test; Molecular Sequence Data; Plasmids; Protein Biosynthesis |
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