PMID:1938875
| Citation |
Nurse, P, Zavitz, KH and Marians, KJ (1991) Inactivation of the Escherichia coli priA DNA replication protein induces the SOS response. J. Bacteriol. 173:6686-93 |
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| Abstract |
Many of the proteins that operate at the replication fork in Escherichia coli have been defined genetically. These include some of the subunits of the DNA polymerase III holoenzyme, the DnaB replication fork helicase, and the DnaG primase. The multiprotein primosome (which includes the DnaB and DnaG proteins), defined biochemically on the basis of its requirement during bacteriophage phi X174 complementary-strand synthesis, could serve as the helicase-primase replication machine on the lagging-strand template. In order to determine if this is the case, we have begun an investigation of the phenotypes of mutants with mutations priA, priB, and priC, which encode the primosomal proteins factor Y (protein n'), n, and n", respectively. Inactivation of priA by insertional mutagenesis resulted in the induction of the SOS response, as evinced by induction of a resident lambda prophage, extreme filamentation, and derepression of an indicator operon in which beta-galactosidase production was controlled by the dinD1 promoter. In addition, the copy numbers of resident pBR322 plasmids were reduced four- to fivefold in these strains, and production of phi X174 phage was delayed considerably. These results are discussed in the context of existing models for SOS induction and possible roles for the PriA protein at the replication fork in vivo. |
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| Keywords |
Bacterial Proteins/genetics; Blotting, Southern; DNA Replication; DNA, Bacterial/biosynthesis; Escherichia coli/genetics; Genes, Bacterial; Mutagenesis, Insertional; Phenotype; SOS Response (Genetics) |
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