PMID:19293938
| Citation |
Jandu, N, Ho, NK, Donato, KA, Karmali, MA, Mascarenhas, M, Duffy, SP, Tailor, C and Sherman, PM (2009) Enterohemorrhagic Escherichia coli O157:H7 gene expression profiling in response to growth in the presence of host epithelia. PLoS ONE 4:e4889 |
|---|---|
| Abstract |
The pathogenesis of enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection is attributed to virulence factors encoded on multiple pathogenicity islands. Previous studies have shown that EHEC O157:H7 modulates host cell signal transduction cascades, independent of toxins and rearrangement of the cytoskeleton. However, the virulence factors and mechanisms responsible for EHEC-mediated subversion of signal transduction remain to be determined. Therefore, the purpose of this study was to first identify differentially regulated genes in response to EHEC O157:H7 grown in the presence of epithelial cells, compared to growth in the absence of epithelial cells (that is, growth in minimal essential tissue culture medium alone, minimal essential tissue culture medium in the presence of 5% CO(2), and Penassay broth alone) and, second, to identify EHEC virulence factors responsible for pathogen modulation of host cell signal transduction. |
| Links |
PubMed PMC2654852 Online version:10.1371/journal.pone.0004889 |
| Keywords |
Cell Line; Epithelial Cells/microbiology; Escherichia coli O157/genetics; Escherichia coli O157/growth & development; Escherichia coli O157/pathogenicity; Gene Expression Profiling; Genes, Bacterial; Humans; Oligonucleotide Array Sequence Analysis; Virulence/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.