PMID:19233928
| Citation |
Gonzalez, MD and Beckwith, J (2009) Divisome under construction: distinct domains of the small membrane protein FtsB are necessary for interaction with multiple cell division proteins. J. Bacteriol. 191:2815-25 |
|---|---|
| Abstract |
Cell division in bacteria requires the coordinated action of a set of proteins, the divisome, for proper constriction of the cell envelope. Multiple protein-protein interactions are required for assembly of a stable divisome. Within the Escherichia coli divisome is a conserved subcomplex of inner membrane proteins, the FtsB/FtsL/FtsQ complex, which is necessary for linking the upstream division proteins, which are predominantly cytoplasmic, with the downstream division proteins, which are predominantly periplasmic. FtsB and FtsL are small bitopic membrane proteins with predicted coiled-coil motifs, which themselves form a stable subcomplex that can recruit downstream division proteins independently of FtsQ; however, the details of how FtsB and FtsL interact together and with other proteins remain to be characterized. Despite the small size of FtsB, we identified separate interaction domains of FtsB that are required for interaction with FtsL and FtsQ. The N-terminal half of FtsB is necessary for interaction with FtsL and sufficient, when in complex with FtsL, for recruitment of downstream division proteins, while a portion of the FtsB C terminus is necessary for interaction with FtsQ. These properties of FtsB support the proposal that its main function is as part of a molecular scaffold to allow for proper formation of the divisome. |
| Links |
PubMed PMC2668415 Online version:10.1128/JB.01597-08 |
| Keywords |
Cell Cycle Proteins/metabolism; Cell Division; Escherichia coli/growth & development; Escherichia coli/physiology; Escherichia coli Proteins/metabolism; Membrane Proteins/metabolism; Protein Interaction Domains and Motifs; Protein Interaction Mapping; Sequence Deletion |
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