PMID:1917868
| Citation |
Rasmussen, LJ, Møller, PL and Atlung, T (1991) Carbon metabolism regulates expression of the pfl (pyruvate formate-lyase) gene in Escherichia coli. J. Bacteriol. 173:6390-7 |
|---|---|
| Abstract |
The anaerobic expression of pfl is reduced both in a strain mutated in the pgi gene and in a pfkA pfkB double mutant strain when cells are grown in medium supplemented with glucose. When cells are grown in medium supplemented with either fructose or pyruvate, no reduction is observed in these strains. The amount of pyruvate in the cells may be responsible for the reduced expression of pfl in the strains mutated in the genes encoding the glycolytic enzymes. Because of the lowered oxygen concentration in the medium, the expression of pfl is induced when an exponentially growing culture enters the stationary phase. This induction is increased when the Casamino Acid concentration is raised 10-fold or when the medium is supplemented with NaCl. Superhelicity of DNA is decreased in a pgi mutant strain grown in medium supplemented with glucose. The superhelicity is also changed, but the opposite way, in a wild-type strain grown in medium supplemented with Casamino Acids at a high concentration or 0.3 M sodium chloride. Our data show that changes in superhelicity do not affect the aerobic expression of pfl but might be important for the anaerobic induction of pfl. |
| Links | |
| Keywords |
Acetyltransferases/genetics; Amino Acids/pharmacology; DNA, Bacterial/physiology; DNA, Superhelical/physiology; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Fructose/metabolism; Fructose/pharmacology; Gene Expression Regulation, Bacterial/physiology; Glucose/metabolism; Glucose/pharmacology; Kinetics; Mutation/genetics; Operon/genetics; Osmolar Concentration; Pyruvates/metabolism; Pyruvates/pharmacology; Pyruvic Acid; Recombinant Fusion Proteins/biosynthesis; Sodium Chloride/pharmacology; beta-Galactosidase/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.