PMID:1911948
| Citation |
Bailone, A, Sommer, S, Knezević, J, Dutreix, M and Devoret, R (1991) A RecA protein mutant deficient in its interaction with the UmuDC complex. Biochimie 73:479-84 |
|---|---|
| Abstract |
recA1730 is a dominant point mutation preventing SOS mutagenesis. We demonstrate here that: i) RecA1730 fails to produce mutagenesis even though UmuD' is formed, ii) recA1730, when complemented by recA+, can cleave LexA protein and it displays a UmuDC- phenotype in spite of adequate concentrations of matured UmuD' and UmuC proteins, iii) the Mut- phenotype caused by RecA1730 is partially alleviated by MucAB proteins, functional analogs of UmuDC. To explain the mutant phenotype, we postulate that recA1730 impairs a RecA function required for the positioning of the UmuD'C complex within the replisome at the site of lesions. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA Damage; DNA-Directed DNA Polymerase; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Mutation; Phenotype; Rec A Recombinases/genetics; SOS Response (Genetics) |
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