PMID:1911947
| Citation |
Bailone, A, Sommer, S, Knezević, J and Devoret, R (1991) Substitution of UmuD' for UmuD does not affect SOS mutagenesis. Biochimie 73:471-8 |
|---|---|
| Abstract |
In order to study the role of UmuDC proteins in SOS mutagenesis, we have constructed new Escherichia coli K-12 strains to avoid i) over-production of Umu proteins, ii) the formation of unwanted mixed plasmid and chromosomal Umu proteins upon complementation. We inserted a mini-kan transposon into the umuD gene carried on a plasmid. The insertion at codon 24 ends protein translation and has a polar effect on the expression of the downstream umuC gene. We transferred umuD24 mutation to the E coli chromosome. In parallel, we subcloned umuD+ umuC+ or umuD' umuC+ genes into pSC101, a low copy number plasmid. In a host with the chromosomal umuD24 mutation, plasmids umuD+ umuC+ or umuD' umuC+ produced elevated resistance to UV light and increased SOS mutagenesis related to a gene dosage of about 3. UV mutagenesis was as high in umuD' umuC+ hosts devoid of UmuD+ protein as in umuD+ umuC+ hosts. UmuD' protein, the maturated form of UmuD, can substitute for UmuD in SOS mutagenesis. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Codon; DNA-Directed DNA Polymerase; Escherichia coli/genetics; Escherichia coli/radiation effects; Escherichia coli Proteins; Gene Expression/radiation effects; Genes, Bacterial/radiation effects; Mutagenesis, Insertional; Plasmids; Protein Biosynthesis; SOS Response (Genetics)/radiation effects; Ultraviolet Rays |
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