PMID:19111659
| Citation |
Luo, X, Hsiao, HH, Bubunenko, M, Weber, G, Court, DL, Gottesman, ME, Urlaub, H and Wahl, MC (2008) Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex. Mol. Cell 32:791-802 |
|---|---|
| Abstract |
Protein S10 is a component of the 30S ribosomal subunit and participates together with NusB protein in processive transcription antitermination. The molecular mechanisms by which S10 can act as a translation or a transcription factor are not understood. We used complementation assays and recombineering to delineate regions of S10 dispensable for antitermination, and determined the crystal structure of a transcriptionally active NusB-S10 complex. In this complex, S10 adopts the same fold as in the 30S subunit and is blocked from simultaneous association with the ribosome. Mass spectrometric mapping of UV-induced crosslinks revealed that the NusB-S10 complex presents an intermolecular, composite, and contiguous binding surface for RNAs containing BoxA antitermination signals. Furthermore, S10 overproduction complemented a nusB null phenotype. These data demonstrate that S10 and NusB together form a BoxA-binding module, that NusB facilitates entry of S10 into the transcription machinery, and that S10 represents a central hub in processive antitermination. |
| Links |
PubMed PMC2627990 Online version:10.1016/j.molcel.2008.10.028 |
| Keywords |
Base Sequence; Crystallography, X-Ray; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Models, Molecular; Molecular Sequence Data; Mutation/genetics; Phenotype; Protein Binding; Protein Multimerization; Protein Structure, Secondary; RNA, Bacterial/metabolism; Ribosomal Proteins/chemistry; Ribosomal Proteins/metabolism; Ribosomes/metabolism; Structure-Activity Relationship; Surface Properties; Terminator Regions, Genetic; Transcription Factors/chemistry; Transcription Factors/metabolism; Transcription, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
