PMID:19038054
| Citation |
Crépin, S, Lamarche, MG, Garneau, P, Séguin, J, Proulx, J, Dozois, CM and Harel, J (2008) Genome-wide transcriptional response of an avian pathogenic Escherichia coli (APEC) pst mutant. BMC Genomics 9:568 |
|---|---|
| Abstract |
Avian pathogenic E. coli (APEC) are associated with extraintestinal diseases in poultry. The pstSCAB-phoU operon belongs to the Pho regulon and encodes the phosphate specific transport (Pst) system. A functional Pst system is required for full virulence in APEC and other bacteria and contributes to resistance of APEC to serum, to cationic antimicrobial peptides and acid shock. The global mechanisms contributing to the attenuation and decreased resistance of the APEC pst mutant to environmental stresses have not been investigated at the transcriptional level. To determine the global effect of a pst mutation on gene expression, we compared the transcriptomes of APEC strain chi7122 and its isogenic pst mutant (K3) grown in phosphate-rich medium. |
| Links |
PubMed PMC2648988 Online version:10.1186/1471-2164-9-568 |
| Keywords |
Animals; Birds/microbiology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Fimbriae, Bacterial/genetics; Fimbriae, Bacterial/metabolism; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genome, Bacterial; Mutation; Oligonucleotide Array Sequence Analysis; Operon; Oxidative Stress; RNA, Bacterial/genetics; Reactive Oxygen Species/metabolism; Regulon; Transcription, Genetic |
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The strain used for microarray studies was APEC chi7122, which has the serotype O78:K80:H9.[1] Construction of the pstCAB(del)::kan mutation is described in Lemarche et al. (2005). Briefly, "A deletion (ΔpstCAB) in the pst operon was obtained by removing an internal EcoRV fragment. A kanamycin resistance cassette, carried on a HincII fragment from pUC71K (40, 57), was then ligated to the EcoRV site, resulting in ΔpstCAB::kan."[1]
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