PMID:19028610
| Citation |
Haegler, K, Mueller, NS, Maccarrone, G, Hunyadi-Gulyas, E, Webhofer, C, Filiou, MD, Zhang, Y and Turck, CW (2009) QuantiSpec--Quantitative mass spectrometry data analysis of (15)N-metabolically labeled proteins. J Proteomics 71:601-8 |
|---|---|
| Abstract |
For relative protein quantitation by mass spectrometry we metabolically labeled E. coli bacteria with (15)N-enriched diets. Proteins extracted from (15)N-labeled and unlabeled E. coli bacteria were mixed, separated by two-dimensional gel electrophoresis and enzymatically digested. The resulting tryptic peptides were analyzed by MALDI mass spectrometry. For the relative protein quantitation we developed fully automated software, QuantiSpec (Quantitative Mass Spectrometry Analysis Software), which uses data from MALDI TOF mass spectrometry and the Mascot database search engine. QuantiSpec detects natural as well as partially or fully labeled peptide isotope distributions. For each identified peptide the (15)N incorporation rate is determined by comparing the experimental to a set of theoretical isotope patterns based on the peptide sequence. Relative quantitation is accomplished by calculating the signal intensity ratios for each (14)N/(15)N peptide pair. |
| Links |
PubMed Online version:10.1016/j.jprot.2008.10.004 |
| Keywords |
Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Nitrogen Isotopes/chemistry; Proteome/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
EcoliWiki Links
See also
References
See Help:References for how to manage references in EcoliWiki.
