PMID:19020502

Citation	Pomerantz, RT and O'Donnell, M (2008) The replisome uses mRNA as a primer after colliding with RNA polymerase. Nature 456:762-6
Abstract	Replication forks are impeded by DNA damage and protein-nucleic acid complexes such as transcribing RNA polymerase. For example, head-on collision of the replisome with RNA polymerase results in replication fork arrest. However, co-directional collision of the replisome with RNA polymerase has little or no effect on fork progression. Here we examine co-directional collisions between a replisome and RNA polymerase in vitro. We show that the Escherichia coli replisome uses the RNA transcript as a primer to continue leading-strand synthesis after the collision with RNA polymerase that is displaced from the DNA. This action results in a discontinuity in the leading strand, yet the replisome remains intact and bound to DNA during the entire process. These findings underscore the notable plasticity by which the replisome operates to circumvent obstacles in its path and may explain why the leading strand is synthesized discontinuously in vivo.
Links	PubMed PMC2605185 Online version:10.1038/nature07527
Keywords	DNA Polymerase III/metabolism; DNA Replication; DNA, Bacterial/metabolism; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Models, Molecular; RNA; RNA, Bacterial/metabolism; RNA, Messenger/metabolism
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