PMID:1886618
| Citation |
Nagano, Y, Matsuno, R and Sasaki, Y (1991) An essential gene of Escherichia coli that has sequence similarity to a chloroplast gene of unknown function. Mol. Gen. Genet. 228:62-4 |
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| Abstract |
The dedB gene of Escherichia coli has sequence similarity to the zfpA gene of the chloroplast chromosome. The functions of dedB and zfpA are unknown. We constructed derivatives of temperature-sensitive polA strains into whose chromosomes a plasmid containing the disrupted dedB gene was integrated by homologous recombination. These strains contained normal and disrupted dedB genes in their chromosomes. We then selected plasmid-segregated strains and found no cells containing the disrupted dedB gene, indicating that disruption of the dedB gene was lethal in polA strains of E. coli. |
| Links | |
| Keywords |
Blotting, Southern; Cell Survival/genetics; Chloroplasts; Escherichia coli/genetics; Genes, Bacterial; Plants/genetics; Plasmids |
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